HLA-ABC Antibody (MHC I)

• Catalog Number: orb1823078
• Category: Antibodies
• Description: Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C). Immunoprecipitation of lactoperoxidase radioiodinated cell surface proteins and surface sialoglycoproteins radiolabeled by sodium metaperiodate/tritiated sodium borohydride technique confirmed the structure gp44,p12 (consisting of a 44kDa glycopeptide linked with a non-glycosylated 12kDa peptide) typical for MHC class I antigen(s) as a structure recognized by Bra23/9. Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human REH cells (a pre-B leukaemia cell line) was used as the immunogen for the HLA-ABC antibody.
• UniProt ID: P30499, P30443, P01889
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Antibody Type: Primary Antibody
• Clone Number: Bra-23/9
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This HLA-ABC antibody is available for research use only.
• Note: For research use only