HLA-A2 mAb (BB7.2), InVivoPure

SKU: orb3127221

Description

The BB7.2 monoclonal antibody is a well-characterized mouse IgG2b antibody that specifically recognizes HLA-A2, a major histocompatibility complex (MHC) class I molecule found in humans. This antibody is widely used in immunological research, transplantation studies, and cancer immunotherapy. BB7.2 binds to a conformational epitope of the HLA-A2 heavy chain, typically in association with β2-microglobulin (β2m). It does not cross-react with other HLA class I alleles, making it a valuable tool for distinguishing HLA-A2+ cells from non-HLA-A2+ populations. HLA-A2 is an important molecule in tumor immunology, as many tumor-associated antigens (e.g., NY-ESO-1, MART-1) are presented by HLA-A2 to cytotoxic T lymphocytes (CTLs). The BB7.2 antibody serves as a critical reagent in screening and validating HLA-A2-restricted T-cell responses in cancer vaccine and T-cell therapy research. The HLA-A2 mAb (BB7.2) can be used in Flow cytometry, immunoprecipitation and Wester Blot, immunohistochemistry and functional blocking assay.

Images & Validation

• Reactivity: Human
• Application Notes:
  Recomended Isotype: Mouse IgG2b k Isotype Control, Recomended Isotype Catalog: orb2568792

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: m IgG2b
• Clone No.: BB7.2
• Immunogen: Papain solubilised HLA-A2
• Target: HLA-A2
• Purity: ≥ 90 % (CGE, reducing conditions) ≤ 10 % aggregates (analytical SEC) Endotoxin level ≤ 2 EU/mg
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Concentration: ≥ 1 mg/ mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HLAA2, anti-HLA Class 1, anti HLA 2, Mouse Anti- Human HLA -A2, Anti-HLA class1 A2, BB7.2.

Quick Database Links

Gene Symbol

HLA-A2

UniProt

S6FW97