HK2 (Hexokinase II) Antibody

SKU: orb1925570

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, KO/KD Validated, WB
• Dilution Range: IHC - 1:400, WB - 1:500-1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B3910EV960X37X47
• Immunogen: This HK2 (Hexokinase II) antibody is generated from a mouse immunized with a recombinant protein between 1-170 amino acids from human HK2 (Hexokinase II).
• Target: HK2 (HGNC:4923)
• Molecular Weight: 102380 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hexokinase-2, 2.7.1.1, Hexokinase type II, HK II, Muscle form hexokinase, HK2

Immunohistochemical analysis of paraffin-embedded Human Skeletal muscle section using Pink1. Diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-HK2 (Hexokinase II) Antibody at 1:500-1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

HK2 (HGNC:4923)

UniProt

P52789