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Item 1 of 5
HK1 (Hexokinase) Antibody (N-term)
Catalog Number: orb1928566
| Catalog Number | orb1928566 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB3921/RB3922 |
| Tested applications | IHC-P, WB |
| Reactivity | Human, Rat |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:2000, WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 102486 Da |
| Target | This HK1 (Hexokinase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from the N-terminal region of human HK1 (Hexokinase). |
| UniProt ID | P19367 |
| NCBI | NP_000179.2, NP_277035.2, NP_277031.1, NP_277033.1, NP_277032.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Hexokinase-1, Brain form hexokinase, Hexokinase ty Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
HK1 Antibody (L93) western blot analysis in MCF-7 cell line lysates (35 ug/lane).This demonstrates the HK1 antibody detected the HK1 protein (arrow).
Western blot analysis of HK1 (arrow) using HK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2).
All lanes: Anti-hHK1-L93 at 1:2000 dilution. Lane 1: C2C12 whole cell lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 102 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes: Anti-HK1 (Hexokinase) Antibody (N-term) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
