Histone H4 (tri methyl K20) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559094
• Category: Antibodies
• Description: Histone H4 (tri methyl K20) Recombinant Rabbit Monoclonal Antibody
• Target: H4C1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised methylpeptide derived from human Histone H4 around the methylation site of tri methyl Lys20 HR(tri methyl K)VL
• UniProt ID: P62805
• MW: 11 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
• Antibody Type: Recombinant Antibody
• Clone Number: 2C12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H4K20me3; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4;
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary ab, dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, Jurkat, BRL, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 11 kDa, Observed MW: 11 kDa.