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Item 1 of 3
Item 1 of 3
| Catalog Number | orb767568 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Histone H4 (phospho-Ser47) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H4 around the phosphorylation site of Ser47. AA range:13-62 |
| MW | 13 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | WB 1:500-2000 Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-HIST1H4A antibody, anti-H4/A antibody, anti-H Read more... |
| Note | For research use only |
Immunofluorescence analysis of HUVEC cells, using Histone H4 (Phospho-Ser47) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
