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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−| Tested Applications | ChIP, DOT, ELISA, FC, ICC, IHC-P, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try; WB: Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa). Can be blocked with Histone H4 peptide - mono methyl K20 |
| Reactivity | A. thaliana, Drosophila, Human, Mammal |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 5E10-D8 |
| Immunogen | Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched)) |
| Target | Histone H4 (mono methyl K20) |
| Purification | Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody (from supernatant) containing PBS + 0.1% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H4F2 antibody;H4FN antibody;HIST1H4 antibody;HIST2H4 antibody;HISTH4H4 antibody;methyl histone H4 antibody;methylated histone H4 antibody
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Quick Database Links
Gene Symbol
Histone H4 (mono methyl K20)
NCBI Gene ID
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
ChIP
Chromatin Immunoprecipitation








