Histone H4 K20me2 antibody

• Catalog Number: orb420429
• Category: Antibodies
• Description: Histone H4 K20me2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, DOT, IF, IHC, WB
• Reactivity: C. elegans, Human
• Isotype: IgG
• Immunogen: Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.
• Concentration: 0.53 mg/ml
• Dilution range: ChIP: 2-5µg/million cells, IHC: 1:50, IF: 1:50, WB: 1:2000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Histone H4 [Dimethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P62805
• NCBI: NP_001029249
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Histone H4 dimethyl Lys20 antibody, H4
• Note: For research use only
• Application notes: Anti-Histone H4 [Dimethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
• Expiration Date: 12 months from date of receipt.