Histone H3.3 Recombinant Rabbit Monoclonal Antibody

SKU: orb559096

Description

Histone H3.3 Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:200-1000, IHC-P=1:100-400, IHC-F=1:100-400, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 7B12
• Immunogen: Recombinant human Histone H3.3 protein, full length.
• Target: H3-3A
• Molecular Weight: 15 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

H3 histone family 3A; H3 histone family 3B; H3 histone, family 3B(H3.3B); H3.3; H3.3A; H3.3B; H33_HUMAN; H3F3; H3F3A; H3f3b; Histone H3.3Q; Histone H3.A; Histone H3.B; MGC87782; MGC87783.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human SY5Y cell lysates, Lane 2: Human MOLT-4 cell lysates, Lane 3: Human THP-1 cell lysates, Lane 4: Human 293T Cell Lysates, Lane 5: Human K562 Cell Lysates, Lane 6: Human U251 Cell Lysates, Lane 7: Human HELA Cell Lysates, Primary: Anti-Histone H3.3 (15kD) (orb559096) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kDa, Observed band size: 15 kDa.

Quick Database Links

Gene Symbol

H3-3A

UniProt

P84243