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Catalog Number | orb765408 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Histone H3.3 |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3.3. AA range:16-65 |
Concentration | 1 mg/ml |
Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 15 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-H3F3A antibody, anti-H3.3A antibody, anti-H3F Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cell. 1, Histone H3.3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH Monoclonal Antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Western Blot analysis of Hela cells using Histone H3.3 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of HeLa cells, using Histone H3.3 Antibody. The picture on the right is blocked with the synthesized peptide.
ELISA, IF, IHC-P | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ChIP, ICC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |