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Histone H3.3 rabbit pAb

Catalog Number: orb765408

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb765408
CategoryAntibodies
DescriptionRabbit polyclonal antibody to Histone H3.3
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human Histone H3.3. AA range:16-65
Concentration1 mg/ml
Dilution rangeImmunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
ConjugationUnconjugated
MW15
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Alternative namesAnti-H3F3A antibody, anti-H3.3A antibody, anti-H3F
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NoteFor research use only
Expiration Date12 months from date of receipt.
Histone H3.3 rabbit pAb

Immunofluorescence analysis of Hela cell. 1, Histone H3.3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH Monoclonal Antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Histone H3.3 rabbit pAb

Western Blot analysis of Hela cells using Histone H3.3 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Histone H3.3 rabbit pAb

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Histone H3.3 rabbit pAb

Immunofluorescence analysis of HeLa cells, using Histone H3.3 Antibody. The picture on the right is blocked with the synthesized peptide.

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