Histone H3 (Tri-Methyl-K5) rabbit pAb

• Catalog Number: orb771705
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3 (Tri-Methyl-K5)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: Synthesized Tri-Methyl peptide derived from human Histone H3. at AA range: K5
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-H3K5ME3 antibody, anti-HIST1H3A antibody, ant
• Note: For research use only

Western Blot analysis of hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour) .