Histone H3 (Tri Methyl K27) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499325
• Category: Antibodies
• Description: Histone H3 (Tri Methyl K27) Recombinant Rabbit Monoclonal Antibody
• Target: H3C1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H3 (Tri Methyl K27)
• UniProt ID: P68431
• MW: 15 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Clone Number: R3H2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3K27me3; Tri-Methyl-Histone H3(Lys27); Histone H3
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2, hours at room temperature, Secondary Ab: 1: HeLa, 2: N2a, 3: BRL, 4: Rat liver, 5: Mouse kidney, 6: Recombinant Histone H3 (20 ng), Protein loading quantity: 20 µg/20 ng, Exposure time: 60 s, Predicted MW: 15kDa, Observed MW: 15kDa.