Histone H3 (phospho Thr11) rabbit pAb

• Catalog Number: orb770562
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3 (phospho-Thr11)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the phosphorylation site of Thr11. AA range:1-50
• MW: 15kD
• Tested applications: ELISA, IF
• Dilution range: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HIST1H3A antibody, anti-H3FA antibody, anti-H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H3 (Phospho-Thr11) Antibody.

Immunofluorescence analysis of HUVEC cells, using Histone H3 (Phospho-Thr11) Antibody. The picture on the right is blocked with the phospho peptide.