Histone H3 (phospho Ser28) rabbit pAb

• Catalog Number: orb764203
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3 (phospho-Ser28)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the phosphorylation site of Ser28. AA range:13-62
• MW: 20
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HIST1H3A antibody, anti-H3FA antibody, anti-H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Western Blot analysis of 293T cells using Phospho-Histone H3 (S28) Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using Histone H3 (Phospho-Ser28) Antibody. The lane on the right is blocked with the phospho peptide.