Histone H3 Antibody (pSer10)

• Catalog Number: orb699714
• Category: Antibodies
• Description: Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clarks level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A synthetic peptide corresponding to the amino acids surrounding phospho S10 were used as the immunogen for the recombinant PHH3 antibody.
• UniProt ID: P68431
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the recombinant PHH3 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PHH3/471R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant PHH3 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human colon with recombinant PHH3 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.