Histone H3 (Acetyl Lys23) rabbit pAb Antibody

SKU: orb763964

Description

Rabbit polyclonal antibody to Histone H3 (Acetyl Lys23)

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23. AA range:-9-40
• Molecular Weight: 17
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-HIST1H3A antibody, anti-H3FA antibody, anti-HIST1H3B antibody, anti-H3FL antibody, anti-HIST1H3C antibody, anti-H3FC antibody, anti-HIST1H3D antibody, anti-H3FB antibody, anti-HIST1H3E antibody, anti-H3FD antibody, anti-HIST1H3F antibody, anti-H3FI antibody, anti-HIST1H3G antibody, anti-H3FH antibody, anti-HIST1H3H antibody, anti-H3FK antibody, anti-HIST1H3I antibody, anti-H3FF antibody, anti-HIST1H3J antibody, anti-H3FJ antibody, anti-Histone H3.1 antibody, anti-Histone H3/a antibody, anti-Histone H3/b antibody

Western Blot analysis of various cells using Acetyl-Histone H3 was diluted at 1:20000.

Western Blot analysis of L929 cells using Acetyl-Histone H3 was diluted at 1:20000.

Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys23) Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys23) Antibody. The lane on the right is blocked with the synthesized peptide.