Histone H3 (Acetyl Lys18) rabbit pAb

• Catalog Number: orb770566
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H3 (Acetyl Lys18)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18. AA range:1-50
• MW: 17
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HIST1H3A antibody, anti-H3FA antibody, anti-H
• Note: For research use only

Western blot analysis of SH-SY5Y lysis using Acetyl-Histone H3 (K18) antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys18) Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys18) Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys18) Antibody. The lane on the right is blocked with the synthesized peptide.