Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559154
• Category: Antibodies
• Description: Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody
• Target: H3C1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K14
• UniProt ID: P68431
• MW: 15 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 1C6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3K14ac; Histone H3(Lys14); H3 histone family memb
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Histone H3 (acetyl K14)) monoclonal Antibody, Unconjugated (orb559154) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Histone H3 (acetyl K14)) monoclonal Antibody, Unconjugated (orb559154) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (acetyl K14)) Monoclonal Antibody, Unconjugated (orb559154) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H3 (acetyl K14)) Monoclonal Antibody, Unconjugated (orb559154) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

PC-3M cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Histone H3 (acetyl K14)) monoclonal Antibody, Unconjugated (orb559154) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Jurkat cell lysate, Lane 2: rat liver tissue lysate, Lane 3: mouse lung tissue lysate, Primary: Anti-Histone H3 (acetyl K14) (orb559154) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 15 kD, Observed band size: 14 kD.