Histone H2A.X (Ab-139) Antibody

• Catalog Number: orb685337
• Category: Antibodies
• Description: Histone H2A.X (Ab-139) Antibody
• Target: H2AFX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: liquid
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Immunogen: Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
• UniProt ID: P16104
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: WB: 1: 500-1: 1000, IHC-P: 1: 50-1: 200, IF: 1: 100-1: 200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-H2A.X antibody, anti-H2AFX antibody, anti-H2a
• Note: For research use only

Immunofluorescence analysis of Hela cells using Histone H2A.X (Ab-139) antibody

Western blot analysis of HepG2 and 293 cells using Histone H2A.X (Ab-139) antibody

Immunohistochemical staining of human breast carcinoma tissue using Histone H2A.X (Ab-139) antibody