Histone H1.3/Histone H1.4(Phospho-Thr17) Rabbit mAb Antibody

SKU: orb615057

Description

Histone H1.3(Phospho-T17)+Histone H1.4(Phospho-T17) Antibody

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Clone No.: SR38-03
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr17 of human H1.4
• Molecular Weight: 30 kDa
• Purity: ProA affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1*TBS (pH7.4), 1%rAlbumin, 40%Glycerol. Preservative: 0.05% Sodium Azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on CRC cell lysates using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody at 1/500 dilution. Positive control: Lane 1: Untreated CRC whole cell lysates Lane2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Histone H1.3(T17)+Histone H1.4(T17) antibody. Counter stained with hematoxylin

ICC staining Phospho-Histone H1.3(T17)+Histone H1.4(T17) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS

Quick Database Links

UniProt

P10412