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| Catalog Number | orb395136 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Histone H1.2. |
| Target | Histone H1.2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | liquid |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Immunogen | histone cluster 1, H1c |
| UniProt ID | P16403 |
| MW | 32-33kd |
| Tested applications | ELISA, IF, IHC, IP, WB |
| Dilution range | WB: 1:500-1:3000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |

L02 cells were subjected to SDS PAGE followed by western blot with orb395136 (HIST1H1C antibody) at dilution of 1:500.

Immunofluorescent analysis of Hela cells, using HIST1H1C antibody orb395136 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-Histone H1.2 (IP: orb395136, 3 ug; Detection: orb395136 1:600) with HeLa cells lysate 1200 ug.

Immunohistochemical of paraffin-embedded human ovary tumor using orb395136 (HIST1H1C antibody) at dilution of 1:100.
ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ChIP, ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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