Histone H1.2 Antibody

• Catalog Number: orb395136
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H1.2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: histone cluster 1, H1c
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:3000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 32-33kd
• Target: Histone H1.2
• UniProt ID: P16403
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

L02 cells were subjected to SDS PAGE followed by western blot with orb395136 (HIST1H1C antibody) at dilution of 1:500.

Immunofluorescent analysis of Hela cells, using HIST1H1C antibody orb395136 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-Histone H1.2 (IP: orb395136, 3 ug; Detection: orb395136 1:600) with HeLa cells lysate 1200 ug.

Immunohistochemical of paraffin-embedded human ovary tumor using orb395136 (HIST1H1C antibody) at dilution of 1:100.