Histone H1.2 Rabbit Polyclonal Antibody

SKU: orb395136

Description

Rabbit polyclonal antibody to Histone H1.2.

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: WB: 1:500-1:3000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: histone cluster 1, H1c
• Target: Histone H1.2
• Molecular Weight: 32-33kd
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

L02 cells were subjected to SDS PAGE followed by western blot with orb395136 (HIST1H1C antibody) at dilution of 1:500.

Immunofluorescent analysis of Hela cells, using HIST1H1C antibody orb395136 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-Histone H1.2 (IP: orb395136, 3 ug; Detection: orb395136 1:600) with HeLa cells lysate 1200 ug.

Immunohistochemical of paraffin-embedded human ovary tumor using orb395136 (HIST1H1C antibody) at dilution of 1:100.

Quick Database Links

Gene Symbol

Histone H1.2

UniProt

P16403