HIST2H2BE/H2BC21 Rabbit Polyclonal Antibody

SKU: orb2954822

Description

Anti-HIST2H2BE/H2BC21 Polyclonal Antibodyis a rabbit polyclonal antibody detecting H2B/q in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Anopheles gambiae, and Asterias rubens. Highlights Affinity Purified: Minimal background and high purity for reliable results. Multi-Application: Validated across multiple applications. Multi-Species: Cross-reactive for translational research.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Bacteria, Bovine, Drosophila, Duck, Fish, Gallus, Human, Mouse, Other, Rat, Xenopus, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E. coli - derived recombinant Human HIST2H2BE/H2BC21 (Pro2-Lys126).
• Purification: Purified by antigen affinity column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H2B/q, Histone H2B-GL105, H2B-clustered histone 21, Histone H2B.q, H2BC21, Histone H2B type 2-E, HIST2H2BE, H2BFQ

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting HIST2H2BE (orb2954822) at a concentration of 13.2 µg/mL overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of HIST2H2BE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Quick Database Links

UniProt

Q16778