HIST1H4A Antibody (C-term)

SKU: orb1788362

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: C. elegans, Drosophila, Other, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This HIST1H4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 71-103 amino acids from the C-terminal region of human HIST1H4A. Antigen Region: 71-103 aa.
• Target: H4C1
• Molecular Weight: 11367 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Histone H4, HIST1H4A, H4/A, H4FA

Immunohistochemical analysis of paraffin-embedded M. skin section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. skin section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. skin section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of MCF-7 cells using HIST1H4A Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded M. esophagus section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. esophagus section using HIST1H4A Antibody (C-term). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from A431, mouse NIH/3T3, L929, rat C6, Hela cell line (from left to right), using HIST1H4A Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Quick Database Links

Gene Symbol

H4C1

UniProt

P62805