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| Catalog Number | orb516312 |
|---|---|
| Category | Antibodies |
| Description | This HIST1H4A (Ab-8) Antibody is an unconjugated polyclonal product. It tagets HIST1H4A using a Peptide sequence around site of Lys (8) derived from Human Histone H4 as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IHC, IP, WB. Purification: Antigen Affinity Purified. |
| Target | HIST1H4A |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
| Purification | Antigen Affinity Purified |
| Immunogen | Peptide sequence around site of Lys (8) derived from Human Histone H4 |
| UniProt ID | P62805 |
| Tested applications | ChIP, ELISA, IF, IHC, IP, WB |
| Dilution range | WB:1:500-1:5000, IHC-P:1:10-1:100, IF:1:50-1:200, IP:1:200-1:2000 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | dJ160A22.1 antibody; dJ160A22.2 antibody; dJ221C16 Read more... |
| Research Area | Epigenetics & Chromatin |
| Note | For research use only |

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- HIST1H4A (Ab-8) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time P

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissueusing HIST1H4A (Ab-8) antibody

Immunoprecipitation analysis of Hela whole cell lysate using HIST1H4A (Ab-8) antibody

Immunofluorescence staining of Hela cells using HIST1H4A (Ab-8) antibody

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using HIST1H4A (Ab-8) antibody
ChIP, ELISA, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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