HIST1H4A (Ab-12) Antibody

• Catalog Number: orb516334
• Category: Antibodies
• Description: HIST1H4A (Ab-12) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Peptide sequence around site of Lys (12) derived from Human Histone H4
• Dilution range: WB:1:500-1:5000, IHC-P:1:20-1:200, IP:1:200-1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIST1H4A
• UniProt ID: P62805
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: dJ160A22.1 antibody; dJ160A22.2 antibody; dJ221C16
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- HIST1H4A (Ab-12) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time

Western blot aanalysis of Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysateusing HIST1H4A (Ab-12) antibody

Immunoprecipitation analysis of Hela whole cell lysate using HIST1H4A (Ab-12) antibody

Immunohistochemistry of paraffin-embedded human colon cancer tissue using HIST1H4A (Ab-12) antibody