HIST1H2AG Antibody (Center)

• Catalog Number: orb1788363
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Drosophila, Other, Rat, Zebrafish
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: 63-87 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 14091 Da
• Target: This HIST1H2AG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-87 amino acids from the Central region of human HIST1H2AG.
• UniProt ID: P0C0S8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (Center) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody (Center). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody (Center). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody (Center). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody (Center). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from mouse NIH/3T3, CEM, Hela, HepG2 cell line (from left to right), using HIST1H2AG Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.