HIST1H1E (Ab-25) Antibody

• Catalog Number: orb416580
• Category: Antibodies
• Description: This HIST1H1E (Ab-25) Antibody is an unconjugated polyclonal product. It tagets HIST1H1E using a Peptide sequence around site of Lys (25) derived from Human Histone H1.4 as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IHC, WB. Purification: Antigen Affinity Purified.
• Target: HIST1H1E
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of Lys (25) derived from Human Histone H1.4
• UniProt ID: P10412
• Tested applications: ChIP, ELISA, IF, IHC, WB
• Dilution range: WB:1:500-5000, IHC-P:1:20-200, IF:1:50-200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Histone H1.4 antibody, anti- Histone H1b anti
• Research Area: Epigenetics & Chromatin
• Note: For research use only

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- HIST1H1E (Ab-25) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time

Western blot aanalysis of HEK293 whole cell lysate, K562 whole cell lysate, Mouse lung tissueusing HIST1H1E (Ab-25) antibody

Immunofluorescent analysis of Hela cells using HIST1H1E (Ab-25) antibody

Immunohistochemistry of paraffin-embedded human colon cancer tissue using HIST1H1E (Ab-25) antibody

Immunohistochemistry of paraffin-embedded human melanoma tissue using HIST1H1E (Ab-25) antibody