HisRS rabbit pAb

• Catalog Number: orb765390
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HisRS
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human HARS. AA range:460-509
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 60
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-HARS antibody, anti-HRS antibody, anti-Histid
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using HisRS Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).