His-Tag Ni-NTA Resin

SKU: orb1173398

Description

His-Tag Ni-NTA Resin utilizes nickel-charged nitrilotriacetic acid on 4% agarose for high-capacity IMAC purification. Featuring four metal-binding sites, this stable resin ensures low leaching and high selectivity, even under harsh conditions involving denaturants or reducing agents.

Research Area

Protein Biochemistry

Images & Validation

• Application Notes:
  His-Tag Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

Storage & Handling

• Storage: Stable for one year at 2-8°C from date of shipment. Do not freeze.
• Form/Appearance: Liquid solution with 50% slurry in 20% ethanol.
• Buffer/Preservatives: Liquid in PBS buffer containing 20% ethanol.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

Ni-NTA; His resin; Ni-NTA resin; His tag resin