His-Tag Protein Purification Kit (Ni-NTA)

SKU: orb1173396

Description

PurKine His-Tag Protein Purification Kit purifies overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in kit consists of 90μm beads of cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled.

Research Area

Protein Biochemistry

Images & Validation

• Application Notes:
  PurKine His-Tag Protein Purification Kit effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in kit consists of 90μm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

Storage & Handling

• Storage: Stable for one year at 2-8°C from date of shipment. Do not freeze.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

Ni-NTA; His resin; Ni-NTA resin; His tag resin