His Tag Antibody

• Catalog Number: orb1787858
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000
• Conjugation: Unconjugated
• MW: 45-50kd
• Target: Poly-HIS peptide were used to produced this antibody.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Poly-His, Hexa-HIS, Hexa-Histidine
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-6×His Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower). Lane 1: 293T/17 transfected with 12tag lysate (10ug). Lane 2: Non-transfected 293T/17 lysate (10ug). Lane 3: 12tag recombinant protein lysate (1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.