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His Tag Antibody

SKU: orb1787858

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

Tested ApplicationsWB
Dilution RangeWB: 1:1000

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenPoly-HIS peptide were used to produced this antibody.
TargetPoly-HIS peptide were used to produced this antibody.
Molecular Weight45-50kd
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Poly-His, Hexa-HIS, Hexa-Histidine

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

His Tag Antibody

All lanes: Anti-6×His Tag Antibody at 1:1000 dilution (upper) or GAPDH (lower). Lane 1: 293T/17 transfected with 12tag lysate (10ug). Lane 2: Non-transfected 293T/17 lysate (10ug). Lane 3: 12tag recombinant protein lysate (1ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

His Tag Antibody (orb1787858)

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