HIS Tag Antibody, HRP Conjugate

SKU: orb1926021

Description

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution range	WB - 1:2000

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Molecular Weight	155541 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	PBS
Disclaimer	For research use only

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Anti-HIS Tag Antibody at 1:2000 dilution + 12-tag protein lysate. Lysates/proteins at 20 µg per lane. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-HIS Tag Antibody at 1:2000 dilution + 12tag recombinant protein lysate. Lysates/proteins at 20 µg per lane. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti- HIS Tag Antibody (conjugated HRP) at 1:2000 dilution + Protein whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 45 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P08581

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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