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Item 1 of 3
Item 1 of 3
HIS Tag Antibody, HRP Conjugate
Catalog Number: orb1926021
| Catalog Number | orb1926021 |
|---|---|
| Category | Antibodies |
| Description | Purified Mouse Monoclonal Antibody (Mab) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 6AT18 |
| Tested applications | WB |
| Isotype | IgG1 |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:2000 |
| Form/Appearance | PBS |
| Conjugation | Unconjugated |
| MW | 155541 Da |
| Target | This HIS Tag antibody is generated from a mouse immunized with recombinant protein. |
| UniProt ID | P08581 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-HIS Tag Antibody at 1:2000 dilution + 12-tag protein lysate. Lysates/proteins at 20 µg per lane. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-HIS Tag Antibody at 1:2000 dilution + 12tag recombinant protein lysate. Lysates/proteins at 20 µg per lane. Predicted band size: 45-50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti- HIS Tag Antibody (conjugated HRP) at 1:2000 dilution + Protein whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 45 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.
