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Item 1 of 3
Item 1 of 3
His Tag (2A2) Mouse mAb
Catalog Number: orb1562825
| Catalog Number | orb1562825 |
|---|---|
| Category | Antibodies |
| Description | His Tag (2A2) Mouse mAb |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Other |
| Form/Appearance | Liquid |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Purification | Affinity Purified |
| Immunogen | Synthetic peptide: HHHHHH conjugated to KLH. |
| Tested applications | ICC, IF, IP, WB |
| Dilution range | WB: 1/1000-1/10000 IF: 1/100-1/500 IP: 1/200 |
| Clone Number | 2A2-4H2-10D8 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | 6 His epitope tag; Hexa His tag; HHHHHH epitope ta Read more... |
| Note | For research use only |
2ug His fusion protein+ Primary antibody dilution at 1) 1:5000. 2) 1:10000.
Immunofluorescence analysis of His Tag (2A2) in 293 cells transfected with a Histag protein using His Tag (2A2) antibody (red), and DAPI (blue).
Immunoprecipitation analysis of Flag Tag (7E1) in 1: untransfected 293 cell lysates 2: transfected 293 cell lysates with Histag fusion protein 3: IP (untransfected 293+antiHis mAb+ Protein G agarose) 4: IP (transfected 293+ normal mouse IgG+Protein G agarose) 5: IP (transfected 293+antiHis mAb+ Protein G agarose).
