Hippocalcin Antibody (N-term)

• Catalog Number: orb1935249
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB0505-0506
• Tested applications: IHC-P, WB
• Predicted Reactivity: Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 22427 Da
• Target: This Hippocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human Hippocalcin.
• UniProt ID: P84074
• NCBI: NP_002134.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Neuron-specific calcium-binding protein hippocalci
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from human brain tissue lysate, using Hippocalcin Antibody (R17). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human brain tissue reacted with Hippocalcin antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.