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Catalog Number | orb1929536 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB8536 |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 69425 Da |
Target | This HIPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-566 amino acids from the C-terminal region of human HIPK4. |
UniProt ID | Q8NE63 |
NCBI | NP_653286.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Homeodomain-interacting protein kinase 4, HIPK4 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HIPK4 C-term in HL-60 cell line lysates (35 ug/lane). HIPK4 (arrow) was detected using the purified Pab.
Western blot analysis of HIPK4 (arrow) using rabbit polyclonal HIPK4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIPK4 gene.
Anti-HIPK4 C-term at 1:1000 dilution + H. testis whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.