HIP2 Antibody (Center)

• Catalog Number: orb1931726
• Category: Antibodies
• Description: HIP2 Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P61086
• MW: 22407 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IHC-P - 1:100, IF - 1:10-50, WB - 1:1000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HIP2, LIG
• Research Area: Cell Biology, Neuroscience
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody (Center). Diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of Hela cell stained with HIP2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HIP2 immunoreactivity is localized to Cytoplasm significantly.

HIP2 Antibody (D124) western blot analysis in 293, mouse C2C12 cell line lysates (35 ug/lane). This demonstrates the HIP2 antibody detected the HIP2 protein (arrow).