Hint1 rabbit pAb

SKU: orb768612

Description

Rabbit polyclonal antibody to Hint1

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human HINT1. AA range:71-120
• Molecular Weight: 28
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-HINT1 antibody, anti-HINT antibody, anti-PKCI1 antibody, anti-PRKCNH1 antibody, anti-Histidine triad nucleotide-binding protein 1 antibody, anti-Adenosine 5'-monophosphoramidase antibody, anti-Protein kinase C inhibitor 1 antibody, anti-Protein kinase C-interacting protein 1 antibody, anti-PKCI-1 antibody

Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HeLa cells, using HINT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using HINT1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using HINT1 antibody.