HIF1Alpha Antibody (C-term)

• Catalog Number: orb1928918
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14563
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 92670 Da
• Target: This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-757 amino acids from the C-terminal region of human HIF1Alpha.
• UniProt ID: Q16665
• NCBI: NP_001521.1, NP_851397.1, NP_001230013.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HIF1Alpha Antibody (C-term) in MDA-MB231 cell line lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.

Western blot analysis of HIF1Alpha Antibody (C-term) in mouse cerebellum tissue lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohis

Confocal immunofluorescent analysis of HIF1Alpha Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).