HIF1Alpha Antibody (C-term)

SKU: orb1928918

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB14563
• Target: This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-757 amino acids from the C-terminal region of human HIF1Alpha.
• Molecular Weight: 92670 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8

Western blot analysis of HIF1Alpha Antibody (C-term) in MDA-MB231 cell line lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.

Western blot analysis of HIF1Alpha Antibody (C-term) in mouse cerebellum tissue lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohis

Confocal immunofluorescent analysis of HIF1Alpha Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-757 amino acids from the C-terminal region of human HIF1Alpha.

UniProt

Q16665

RefSeq (Protein):NP_001521.1, NP_851397.1, NP_001230013.1