HIF1A Antibody (N-term)

SKU: orb1930823

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Hamster, Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB11679
• Immunogen: This HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIF1A. Antigen Region: 1-30 aa.
• Target: HIF1A {ECO:0000303|PubMed:7539918, ECO:0000312|HGNC:HGNC:4910}
• Molecular Weight: 92670 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8

Fluorescent confocal image of Hela cell stained with HIF1A Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIF1A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).HIF1A immunoreactivity is localized to cytoplasm and nucleus significantly.

HIF1A Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF1A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of HIF1A Antibody (N-term) in CHO cell line lysates (35 ug/lane). HIF1A (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

HIF1A {ECO:0000303|PubMed:7539918, ECO:0000312|HGNC:HGNC:4910}

UniProt

Q16665

RefSeq (Protein):NP_001521.1, NP_851397.1, NP_001230013.1