HIF-1 Alpha Recombinant Rabbit mAb

SKU: orb2561593

Description

HIF-1 Alpha Recombinant Rabbit mAb

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 4H12
• Immunogen: A synthesized peptide derived from human HIF 1 alpha (616-674/826aa)
• Target: HIF1A
• Molecular Weight: 120 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% Proclin300 and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A.

Western blot analysis of Ramos cell lysate. Using HIF-1 Alpha (orb2561593) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Quick Database Links

Gene Symbol

HIF1A

UniProt

Q16665