HIF-1 alpha Antibody

SKU: orb1326955

Description

Mouse monoclonal antibody to HIF-1 alpha (HIF1A)

Images & Validation

• Tested Applications: WB
• Dilution range: WB 1:500
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: OTI2D5
• Immunogen: Human recombinant protein fragment corresponding to amino acids 531-826 of human HIF1a (NP_001521) produced in E.coli.
• Target: HIF-1 alpha
• Molecular Weight: 92.5 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF1A ( Right lane) using HIF-1 alpha (HIF1A) antibody

Quick Database Links

Gene Symbol

HIF-1 alpha

UniProt

Q16665

RefSeq (mRNA):NM_001530