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Description
Research Area
Cancer Biology
Images & Validation
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Key Properties
−| Source | (BTI-Tn-5B1-4) High-5 Insect cells |
|---|---|
| Biological Activity | Biological activity was determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/mL. Endotoxin level is less than 0.1 ng per μg of HGF (1EU/μg). |
| Target | Hgf |
| Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses. |
| Protein Sequence | Alpha chain: QKKRRNTLHE FKKSAKTTLT KEDPLLKIKT KKVNSADECA NRCIRNRGFT FTCKAFVFDK SRKRCYWYPF NSMSSGVKKG FGHEFDLYEN KDYIRNCIIG KGGSYKGTVS ITKSGIKCQP WNSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG PMDHTESGKT CQRWDQQTPH RHKFLPERYP DKGFDDNYCR NPDGKPRPWC YTLDPDTPWE YCAIKTCAHS AVNETDVPME TTECIQGQGE GYRGTSNTIW NGIPCQRWDS QYPHKHDITP ENFKCKDLRE NYCRNPDGAE SPWCFTTDPN IRVGYCSQIP KCDVSSGQDC YRGNGKNYMG NLSKTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNKNYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR Beta chain: VVNGIPTQTT VGWMVSLKYR NKHICGGSLI KESWVLTARQ CFPARNKDLK DYEAWLGIHD VHERGEEKRK QILNISQLVY GPEGSDLVLL KLARPAILDN FVSTIDLPSY GCTIPEKTTC SIYGWGYTGL INADGLLRVA HLYIMGNEKC SQHHQGKVTL NESELCAGAE KIGSGPCEGD YGGPLICEQH KMRMVLGVIV PGRGCAIPNR PGIFVRVAYY AKWIHKVILT YKL |
Storage & Handling
−| Storage | The lyophilized HGF recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted HGF stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing HGF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SF, NK1, NK2, HGF/SF, SF/HGF, C230052L06Rik, Hepatocyte growth factor, Hepatopoietin-A, SF
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001276387.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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