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Catalog Number | orb1787934 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | 134-162 aa |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100 |
Conjugation | Unconjugated |
MW | 45080 Da |
Target | This HFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-162 amino acids from the Central region of human HFE2. |
UniProt ID | Q6ZVN8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Hemojuvelin, Hemochromatosis type 2 protein, RGM d Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HFE2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HFE2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-HFE2 Antibody (Center) at 1:1000 dilution. Lane 1: human heart lysate. Lane 2: human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.