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Catalog Number | orb1930291 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB24640 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Drosophila, Other, Zebrafish |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 35808 Da |
Target | This HEY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-91 amino acids from the N-terminal region of human HEY2. |
UniProt ID | Q9UBP5 |
NCBI | NP_036391.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Hairy/enhancer-of-split related with YRPW motif pr Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HEY2 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HEY2 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
HEY2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (15 ug/lane). This demonstrates the HEY2 antibody detected HEY2 protein (arrow).
All lanes: Anti-HEY2 Antibody (N-term) at 1:1000 dilution. Lane 1: rat heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 41kDa. Blocking/Dilution buffer: 5% NFDM/TBST.