Hexokinase 1 Rabbit mAb [KD Validated]

• Catalog Number: orb1951657
• Category: Antibodies
• Description: Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Hexokinase 1
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:200
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P19367
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HK1;HK1-ta;HK1-tb;HK1-tc;HKI;HXK1; Hexokinase 1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Hexokinase 1 Antibody.

Western blot analysis of Hexokinase 1 expression in (1) MCF-7 cell lysate; (2) 293T cell lysate.