HK1 Antibody

SKU: orb1239714

Description

HK1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1.The immunogen is located within amino acids 830 - 880 of Hexokinase 1.
• Target: HK1
• Molecular Weight: Predicted: 101 kDa Observed: 103 kDa
• Purification: Hexokinase 1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Hexokinase 1 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HK, HXK

Western blot analysis of Hexokinase 1 in rat bladder tissue lysate with Hexokinase 1 antibody at (A) 1 and (B) 2 µg/ml.

Immunohistochemistry of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 5 µg/mL.

Immunofluorescence of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

HK1

UniProt

P19367

RefSeq (Protein):NP_000179