Hexanoyl-Lysine adduct Antibody: HRP

• Catalog Number: orb396563
• Category: Antibodies
• Description: Mouse monoclonal antibody against Hexanoyl-Lysine adduct conjugated to HRP.
• Target: Hexanoyl-Lysine adduct
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: HRP
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Purification: Protein G Purified
• Immunogen: Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
• Tested applications: ELISA, IHC, WB
• Dilution range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50)
• Application notes: A 1:1000 dilution of SMC-509 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Clone Number: 500000000
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Hexanoyl-Lysine adduct antibody, HEL (Hexanoyl-Lys
• Note: For research use only

Immunohistochemistry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:26 for 2 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 489. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Hexanoyl-Lysine adduct Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 μM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alexa Fluor 633 F-Actin stain. (E, F) Hexanoyl-Lysine adduct Antibody. (G, H) Composite.

Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.

Flow Cytometry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG.