Hexahydrocurcumin

SKU: orb1218106

Description

Hexahydrocurcumin is a selective, orally active COX-2 inhibitor and inactive against COX-1.

Key Properties

• CAS Number: 36062-05-2
• MW: 374.43
• Purity: >98% (HPLC)
• Formula: C₂₁H₂₆O₆
• SMILES: O=C(CC(O)CCC1=CC=C(O)C(OC)=C1)CCC2=CC=C(O)C(OC)=C2
• Target: COX
• Solubility: DMSO:45 mg/mL (120.19 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Hexahydrocurcumin (50 mg/kg; oral administration; daily; for 16 weeks; male Wistar rats) treatment significantly reduces the numbers of aberrant crypt foci (ACF) in colon cancer rats. Hexahydrocurcumin also markedly decreases COX-2 protein expression. The levels of COX-1 protein is not different from normal rats. Animal model: Male Wistar rats (100-120 g) injected with dimethylhydrazine (DMH). Dosage: 50 mg/kg. Administration: Oral administration; daily; for 16 weeks. Result: Significantly reduced the numbers of ACF in colon cancer rats. Also markedly decreased COX-2 protein expression.
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• In Vitro:
  Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) treatment significantly decreased the viability of HT-29 colon cancer cells in a time- and concentration-dependent. The respective IC50 values for 24 and 48 h of Hexahydrocurcumin exposureare 77.05 and 56.95, respectively. Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) combined with 5-fluorouracil (5-FU; 5 μM) markedly reduces the COX-2 expression. The level of COX-1 is not altered. Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) combined with 5-fluorouracil (5-FU; 5 μM) markedly reduces the COX-2 protein. The level of COX-1 protein is not altered. Hexahydrocurcumin (7-14 μM; 24 hours) attenuates lipopolysaccharide (LPS)-elicited increase of prostaglandin E2 (PGE2) in murine macrophages (RAW 264.7) in a concentration-dependent manner. Cell Viability Assay Cell line: HT-29 cells. Concentration: 0 μM, 5 μM, 10 μM, 25 μM. Incubation time: 24 hours or 48 hours. Result: Significantly decreased the viability of HT-29 colon cancer cells. RT-PCR Cell line: HT-29 cells. Concentration: 25 μM. Incubation time: 24 hours. Result: Combined with 5-fluorouracil (5-FU; 5 μM) markedly reduced the COX-2 expression. Western blot analysis. Cell line: HT-29 cells. Concentration: 25 μM. Incubation time: 24 hours. Result: Combined with 5-fluorouracil (5-FU; 5 μM) markedly reduced the COX-2 protein.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

COX