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Item 1 of 6
Item 1 of 6
HEXA Antibody (N-term)
Catalog Number: orb1929694
| Catalog Number | orb1929694 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB20977 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P-Leica: 1:500, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 60703 Da |
| Target | This HEXA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-170 amino acids from the N-terminal region of human HEXA. |
| UniProt ID | P06865 |
| NCBI | NP_000511.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Beta-hexosaminidase subunit alpha, Beta-N-acetylhe Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
HEXA Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of paraffin-embedded Human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Western blot analysis of HEXA Antibody (N-term) in HepG2 cell line lysates (35 ug/lane). HEXA (arrow) was detected using the purified Pab.
Western blot analysis of HEXA Antibody (N-term) in mouse liver tissue lysates (35 ug/lane). HEXA (arrow) was detected using the purified Pab.
Anti-HEXA Antibody (N-term) at 1:1000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-HEXA Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
