Hesperidin

SKU: orb1309991

Description

Hesperidin (Cirantin) is a citrus-derived flavanone glycoside with documented antioxidant, antitumor, and antiallergic properties. It is widely used in research areas including inflammation, cancer, and metabolic studies, employing both in vitro assays and in vivo animal models.

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 520-26-3
• MW: 610.56
• Purity: 99.04% (May vary between batches)
• Formula: C₂₈H₃₄O₁₅
• SMILES: O=C1C=2C(O[C@@H](C1)C3=CC(O)=C(OC)C=C3)=CC(O[C@@H]4O[C@H](CO[C@H]5[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O5)[C@@H](O)[C@H](O)[C@H]4O)=CC2O
• Target: Endogenous Metabolite,Autophagy,Reactive Oxygen Species,ROS,Apoptosis
• Solubility: DMSO:245 mg/mL (401.27 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:6 mg/mL (9.83 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  A549 cells:> 100 μM|Monocyte:> 200 μM|DLD-1 cells:> 100 μM|Antihypertensive agent with lipid-lowering:116.68 μmo/L|H9 cells:> 164 μM|HT29 cells:142 μM
• In Vivo:
  METHODS: To assay antitumor activity in vivo, Hesperidin (200 mg/kg) was administered by gavage to BALB/c mice bearing CT-26 xenografts once daily for five days. Hesperidin was then administered as a single dose of cyclophosphamide (25 mg/kg) for fourteen days. RESULTS: Leukocyte counts were increased in mice treated with Hesperidin prior to cyclophosphamide injection. This significant protective effect was observed 4 and 7 days after cyclophosphamide injection. In CT-26 tumor-bearing mice, co-administration of Hesperidin and cyclophosphamide significantly inhibited cyclophosphamide-induced delay in tumor growth.
• In Vitro:
  METHODS: Tumor cells HeLa and HT-29 were treated with Hesperidin (20-100 µM) for 24-72 h. Cell viability was measured by MTT assay. RESULTS: Treatment with Hesperidin significantly reduced cell viability and the effect of Hesperidin on cell viability was concentration and time dependent. Cells incubated with 100 µM Hesperidin for 72 h showed the greatest antiproliferative effect, with cell viability decreasing to 12% of control cells. METHODS: Human mesothelioma cells MSTO-211H were treated with Hesperidin (40-160 µM) for 48 h. Apoptosis was detected using DAPI and PI staining. RESULTS: The percentage of sub-G1 phase increased from 20% to 35% in 40-160 µM Hesperidi-treated MSTO-211H cells.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

citrus, Cirantin, Apoptosis, antioxidant, antitumor, antiallergic, Autophagy, inhibit, inflammatory, glycoside, flavanone, EndogenousMetabolite, Endogenous Metabolite, Inhibitor, Hesperetin 7-rutinoside, Hesperidin, ReactiveOxygenSpecies, Reactive Oxygen Species

Compound Identifiers

PubChem CID

10621